Prevalence of Escherichia coli in urinary tract infection among patients attending Umaru Sanda Ndayako General Hospital Bida, Niger State, Nigeria.

Abstract

This study was carried out to investigate the prevalence of Escherichia coli in urinary tract infection among the patients attending Umaru Sanda Ndayako General Hospital, Bida. Samples were collected and diagnosed within the period of two months, from May- July, 2013. Growth on MacConkey agar was sub cultured on eosin methylene blue for identification of colonial characteristics of E. coli and other associated microorganisms. One hundred (100) urine samples were screened and 83 samples had significant bacteriuria. Escherichia coli, after standard laboratory urine culture using MacConkey agar and Eosine methylene blue coupled with biochemical tests such as Indole, Methyl red- Vogesproskauer test, and citrate utilization were used as confirmatory tests accounts for 36(40%). Out of the 36(43%) positive E. coli isolated 29(80%) were sensitive to ciprofloxacin, Trimethoprim, chloramphenicol and nitrofurantion. Apart from E. coli, other microorganism sensitive to these antibiotics include, Enterobacter sp, Proteus mirabilis and Klebsiella sp. 7(19.4%) of E. coli, 5(50%) of Proteus mirabilis and 3(33%) Klebsiella sp were resistant to ceporex, Amoxicilline and Tetracycline. Forty-nine (49) (59%) positive cases of UTI were for female and male patient had 34(40%). During this research, middle age women from 21-30 (26.5%) and 31-40(18%) were found to possess the highest prevalence of UTI. The result of this study indicated that E. coli is the most prevalent organism responsible for UTI due to the possession of some virulent associated properties which enable them to adhere to the urinary tract. Appropriate measures should be taken to prevent further spread of this infection most especially in women and children.