Antioxidant Activity of the Woody Root Extracts of Dalbergia saxatilis Hook F. using UV/Visible Spectrophotometer

Abstract

The antioxidant potentials of Dalbergia saxatilis were investigated using 1,1-diphenyl-2-picarylhydrazyl (DPPH) and Vitamin C. The dried pulverized woody root was extracted with 95% ethanol. It was weighed using Metler H10 into test tubes and various concentrations were made. A known concentration of DPPH was carefully added to all the test tubes and allowed to stand for 15 minutes. The test samples were duplicated summing up to thirty test tubes and each of the test tube samples was analyzed using the UV/Visible double beam spectrophotometer. The average values for each test samples were recorded for the crude 95% ethanol and water extract. Percentage inhibition values was calculated and it was clearly shown from the calculated values of the 95% ethanolic extract of the woody root of Dalbergia saxatilis has a high antioxidant activity of (42.9-63.4%) and the water extract has a low antioxidant activity of (26.3-56.2%) when compared with vitamin C (74.2-74.9%). On the basis of the available data in this report, it can be postulated that Dalbergia saxatilis woody root could be used as alternative plant for rich antioxidant.